Coding

Part:BBa_K1039024:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


PhiC31 integrase under lock and key control with lacI expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal XhoI site found at 1519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3619
    Illegal SapI site found at 3133
    Illegal SapI.rc site found at 2824
    Illegal SapI.rc site found at 2882
    Illegal SapI.rc site found at 2987


Design Notes

PhiC31 integrase, base 52 is changed from T to C, silent mutation.


Source

The parts were synthesized from BioBasic Inc or obtained from the iGEM 2013 distribution kit.

References